Team:WHU-China/Notebook

• Dealing with biobricks
• Preparation of competent cells (Using Calcium Chloride)
• Plasmid Extraction（Using Plasmid Mini Kit）
• Transformation (heat-shock method)
• Cleavage with two restriction enzymes
• Get Extraction
• Identification of DNA by Electrophoresis
• Ligation
• PCR of the colony
• Identification of DNA by Sequencing
• Others............

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