Team:KIT-Kyoto/ぷろとこる英語

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いんぐりっしゅ！

Ligation

Refer to following table, prepare a reaction solution.

insert	0.5 µl
vector	0.5 µl
10 x Buffer	2.5 µl
T4 ligase	0.5 µl
H₂O	1.0 µl
	total 5 µl

Incubate it for 30min at 16 ℃.

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