IIT Madras

And then the E.coli said, "Let there be light"

Transformation

• Add 2 ul of Plasmid DNA to competent cells (100 ul aliquot) on ice for 30 minutes.
• Heat shock : 90 s for 43 C.
• Keep on ice for 2 minutes.
• Add 900 ul of LB broth (sterile) into each microfuge tubes.
• Incubate in shaker at 37 C for 1 hour.
• Plate on plates (with antibiotics if necessary) and incubate at 37 C overnight.

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