Team:Peking S/lab/notebook/myr

From 2011.igem.org

6.24-7.5 Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion. 7.6-7.7 Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing. 7.8-7.12 Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome. 7.13-7.23 Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters. 7.24-8.13 Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules. 8.14-9.5 Testing A-factor system performance. 9.6-9.18 Construction cinI/cinR system mainly contains constitutively expressed cinR and a gfp controlled by cinR and cinI synthesized chemical molecules. 9.19-10.1 Characterization of cinI/cinR system.