Materials

Strain(Fruits Fly)

Name	Genotype and/or Relevant Features	Source or Reference
D.melanogaster(fly)	Oregon-R-C	DGRC[http://kyotofly.kit.jp/cgi-bin/stocks/search_res_det.cgi?DB_NUM=1&DG_NUM=105669]

Name	Genotype and/or Relevant Features	Source or Reference
JM109	endA, recA1, gyrA96,thi,hsdR17,(r_κ^-,m_κ⁺),relA1, supE44, λ^-,Δ(lac-proAB),[F',traD36,proAB,lacl^qZΔM15]	Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]

Name	Genotype and/or Relevant Features	Source or Reference
TOP10	F-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16 rpsL(Str^R), endA1, λ-	Invitrogen
KRX	[F′, traD36, ΔompP, proA⁺B⁺, lacI^q, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nal^r), thi-1, hsdR17 (r_k^–, m_k⁺), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.	Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University
KRX		(Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1698])
BL21(DE3)pLysS	F–, ompT, hsdS_B (r_B^–, m_B^–), dcm, gal, λ(DE3), pLysS, Cm^r.	Promega [http://www.promega.com/catalog/catalogproducts.aspx?categoryname=productleaf_1005]

Name	Source or Reference
Lambda Phage (cI857 Sam7)	DNA Digested Markers (#3403 λ-Hind III digest) from Takara Bio [http://catalog.takara-bio.co.jp/en/product/basic_info.asp?unitid=U100005399]
Lambda Phage (cI857 Sam7)	http://www.ncbi.nlm.nih.gov/nuccore/NC_001416

Date	Name	Sequence
07/21	S Forward (Same as SRRz Forward)	gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga
07/21	S Reverse	CTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc
07/21	SRRz Reverse	CTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc
07/28	ΔTMD1 Forward	cgcggcagatataatggcggt
07/28	ΔTMD1 Reverse	gtcatgtttttctggcatcttcatgtct
08/19, 08/24	Sam7 Forward	ggttcattcgtgaccttctcgacttc
08/19, 08/24	Sam7 Reverse	aggcgataatggcgcacatc
08/20	SRRz Forward Retry	gGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg
08/20	SRRz Reverse Retry	CTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg
08/23, 08/24	Deletion of Upstream Forward	gagcaaatccccttattgggggtaagacatg
08/23, 08/24	Deletion of Upstream Reverse	ctctagaagcggccgcgaattcc
09/13, 09/22	Add J23105 Forward	CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
09/13, 09/22	Add J23105 Reverse	AGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc
10/10	Deletion of GFP Forward (Primer1)	ccaggcatcaaataaaacgaaaggctc
10/10	Deletion of GFP-DT Forward (Primer2)	tactagtagcggccgctgc
10/10	Deletion of GFP, GFP-DT Reverse (Primer3)	ctctagtattattgatttctaccatcttctactcc
-	VF2 (<partinfo>G00100</partinfo>)	tgccacctgacgtctaagaa
-	VF (<partinfo>G00101</partinfo>)	attaccgcctttgagtgagc