The Rutgers iGEM Team designed two complex genetic circuits, Etch-a-Sketch and Full Adder, and created a software tool, MYS!S. The Etch-a-Sketch circuit enables a lawn of bacteria to be drawn on with a laser. This seemingly inconsequential task presents many engineering challenges: the bacteria need to be sensitive in order to respond to a laser pulse, yet selective to use in ambient lighting. The second circuit allows bacteria to emulate a digital full adder. The circuit makes use of individually non-functional split reporters that can reform functional reporters with the help of fused “zipper” domains. In addition to the circuit, we have made easily fuse-able biobricks of these domains in order to facilitate the engineering of more split proteins, which should assist in the creation of logic circuits. MYS!S aims to improve the parts registry by checking and giving directions to modify Biobricks to conform to assembly standards.
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The Etch-a-Sketch project aims to create a lawn of bacteria that can be drawn on with a laser pointer. This seemingly inconsequential task actually presents many interesting engineering challenges. In particular, the bacteria need to be extremely sensitive in order to respond to a short light pulse from a laser, but they still must be “selective” enough to use in ambient lighting.
We have designed a novel genetic switch that we hope will tackle these problems. If our work will serve as a useful model for future projects that require massive signal amplification. In particular, researchers creating biosensors may find our work very helpful.
| The Full Adder project seeks to create bacteria that can mimic a digital full adder. This seemingly complex problem can be greatly simplified if we use a certain simple “encoding” on the outputs of the full adder. The full adder incorporates different types of AND gates, including one that incorporates split protein interactions with zipper linker domains. The zipper linker domain is a novel addition to the iGem registry, and by allowing proteins to be split and reassociated promises to bring new possibilities to future iGem projects.
Our insights may prove useful to any genetic engineer or synthetic biologist working on highly complex systems. The zipper linker domains create possibilities of new AND interactions, making complexity in future biologic circuits simple. The bacterial full adder may very well become the ancestor to more complicated biological calculators in the future.
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A major problem with the current Parts Registry, a library of BioBricks submitted by iGEM teams, is that many parts do not strictly conform to the BioBrick standard which makes certain operations extremely difficult. Rutger's iGEM software team strives to provide a tool to improve the standard parts registry by checking, and if need be modifying, the BioBrick parts.
The basic idea is that before a team submits their new BioBrick, it will run the genetic sequences through MYS!S. MYS!S will output the modified genetic sequence, BioCoder source code, and the lab protocol needed to change the unmodified sequence into the modified sequence The long term goal of the project is to further the automation of lab protocols by specifying them through algorithms.
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