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GaTech 2010 Biobrick BBa_k41000
Objectives:
1. Test the BioBrick operation to different substrates and temperatures:
a. Substrates:
i. LB
ii. Minimal essential medium
iii. Saline solution
b. Temperatures:
i. 8°C
ii. 23°C
iii. 37°C
2. Improve the characterization of the BioBrick tested in other substrates to study the performance of the promoter.
3. Define the Biobrick promoter strength at the temperatures and selected substrates.
Methodology
1. We used nine falcon tubes of 25 mL. Three tubes for every different substrate.
2. To each tube we added 10 ml of liquid LB, 10 mL of chloramphenicol (33μg/mL) and 500 µL of K2NO3 40 mM.
3. Tubes corresponding the different substrates were inoculated with BBa_K381001.
4. Tubes were incubated at 37, 8 and 23 ° C overnight.
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