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Team:Virginia Tech/Notebook
From 2011.igem.org
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Notebook
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
Week of 5/23/2011
Design Team: Started collecting sequences of relevant fluorescent proteins and entering them into GenoCAD. Learned about attribute grammars.
Fabrication Team: blah
Characterization Team: blah
Week of 5/30/2011
Design Team: Created first draft of attribute grammar. Collected more fluorescent proteins for GenoCAD. Started looking at modeling aspect of project - explored modeling software that can handle SBML (Systems Biology Markup Language) input - Matlb Simbiology looks nice.
Fabrication Team: blah
Characterization Team: blah
Week of 6/6/2011
Design Team: Small changes to grammar (fixing stop codon issues) and big changes to grammar (changing how parts are ordered and thus how GenoCAD constructs the sequence of a construct we make). Looked further into modeling, how our attribute grammar will connect to that. Collected more fluorescent proteins, started to look into degradation tags and linker sequences for fusion proteins.
Fabrication Team: blah
Characterization Team: blah
Week of 6/13/2011
Design Team: Started working on Prolog compiler which will take a construct from GenoCAD and use it to write a Java file, which will in turn write an SBML file for modeling (this is a good way to do it, we promise).
Fabrication Team: blah
Characterization Team: blah
Week of 6/20/2011
Design Team: blah blah blah
Fabrication Team: blah
Characterization Team: blah
Week of 6/27/2011
Design Team: blah blah blah
Fabrication Team: blah
Characterization Team: blah
Week of 7/4/2011
Design Team: blah blah blah
Fabrication Team: Hayley worked on mCitrine insert in pYES2 backbone and mCherry (E2060) insert in pSB1AK3 backbone with Lac promotor and RBS binding site (J04500). Loran worked on mCherry insert in pYES2 backbone, Plasmid Prep of BioBrick mCherry, and CFP (E0020) inserts. Mandy worked on CFP insert in pSB1AK3 backbone and mCherry insert in PYES2 backbone. Martha worked on GFPmut3b in pSB1AK3 backbone. Swetha worked on mCitrine insert in pYES2 backbone and Degradation Tags Primer Design.
Characterization Team: blah
Week of 7/11/2011
Design Team: blah blah blah
Fabrication Team: Loran worked on mCherry-pYES2, non-Biobrick AcGFP-pYES2, and Biobrick AcGFP-pSB1AK3. Swetha worked on mCitrine-pYES2,Biobrick CFP-pYES2, and Biobrick GFPmut3b-pYES2. Mandy worked on CFP-pSB1AK3, mCherry-pYES2,Biobrick mCherry-pYES2, Biobrick mCherry-pSB1AK3, and Biobrick GFPmut3b-pSB1AK3. Hayley worked on mCitrine-pYES2, Biobrick mCherry-pSB1AK3, and non-Biobrick mCitrine-pSB1AK3. Martha worked on Biobrick GFPmut3b-pSB1AK3.
Characterization Team: blah
Week of 7/18/2011
Design Team: blah blah blah
Fabrication Team: blah
Characterization Team: blah
Week of 7/25/2011
Design Team: blah blah blah
Fabrication Team: blah
Characterization Team: blah
Week of 8/1/2011
Design Team: blah blah blah
Fabrication Team: blah
Characterization Team: blah
Week of 8/8/2011
Design Team: blah blah blah
Fabrication Team: blah
Characterization Team: blah
