Team:Panama/7 June 2011
From 2011.igem.org
we prepared: 500ml LB medium Autoclaving LB medium Glycerol 30% for preservation Check the medium with plasmids, colonies grows well; negative control doesn’t grow after 22h on incubation. We inoculate bacterias with plasmids P+R, R+T and with Rh in liquid LB + Amp. Incubate at 37oC in shaker.