"

From 2011.igem.org

Attributions

References

• Awram, P., and J. Smit. 1998. The Caulobacter crescentus paracrystalline S-layer protein is secreted by an ABC transporter (type I) secretion apparatus. J. of Bacteriol. 180(12):3062-69.
• Bingle, W.H., J.F. Nomellini, and J. Smit. 2000. Secretion of the Caulobacter crescentus S-layer protein: further localization of the C-terminal secretion signal and its use for secretion of recombinant proteins. J. of Bacteriol. 182(11):3298-301.
• Kaplan, J., C. Ragunath, N. Ramasubbu, and D. Fine. 2003. Detachment of Actinobacillus actinomycetemcomitans Biofilm Cells by an Endogenous beta-Hexosaminidase Activity. J. of Bacteriol. 185(16):4693-4698.
• Lu, T., and J. Collins. 2007. Dispersing biofilms with engineered enzymatic bacteriophage. PNAS 104(27):11197-11202.
• Malakooti, J., S. Wang, and B. Ely. 1995. A Consensus Promoter Sequence for Caulobacter crescentus Genes Involved in Biosynthetic and Housekeeping Functions. J. of Bacteriol. 177(15):4372-4376.
• Manuel, S.G.A., C. Ragunath, H. Sait, E. Izano, J. Kaplan, and N. Ramasubbu. 2007. Role of active-site residues of dispersin B, a biofilm-releasing β-hexosaminidase from a periodontal pathogen, in substrate hydrolysis. FEBS J. 274:5987-5999.
• Meisenzahl, A., L. Shapiro, U. Jenal. 1997. Isolation and Characterization of a Xylose-Dependent Promoter from Caulobacter crescentus. J. of Bacteriol. 179(3):592-600.
• Nomellini, J., C. Li, D. Lavallee, I. Shanina, L. Cavacini, M. Horwitz, and J. Smit. 2010. Development of an HIV-1 Specific Microbicide Using Caulobacter crescentus S-Layer Mediated Display of CD4 and MIP1α. PLoS ONE 5(4):e10366.
• Simões, M., L. Simões, and M.J. Vieira. 2010. A review of current and emergent biofilm control strategies. Food Sci. and Tech. 43:573-583.

Attributions and Contributions

We would like to thank the University of British Columbia for providing a copy of the wild type dspB gene. We would also like to thank Dr. Lisa Bowers for her enduring support, guidance, and teaching us our way around the lab. Also thank you to Carolyn Bosse, Kathy Miller, and the Grinnell College Biology Department for providing us with access to instruments and materials for our project. We thank Integrated DNA Technologies (IDT) for generously supporting us with oligos and for synthesizing our two genes, esp and dspB, with codons optimized for expression in Caulobacter.