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Team:Wisconsin-Madison/projectoverview
From 2011.igem.org
Project >>
Overview,
Ethanol Sensor,
Alkane Sensor,
Microcompartment
Project Overview
The UW-Madison iGEM team is working on a biofuel biosensor project for 2011. This project ultimately breaks down into several smaller projects, each described in greater detail in their respective sections: an ethanol sensor, an alkane sensor, and a directed evolution construct for each sensor. We originally intended to use bacterial microcompartments (BMCs)to improve the efficacy of our sensors, however, due to time constraints the project was dropped. Our biosensing systems are designed to produce a red fluorescent protein (RFP) in the presence of whatever they are designed to sense; in our case, either ethanol (EtOH) or n-alkanes. These two compounds are frequently used for biofuel. Currently, common laboratory chromatography techniques are used to characterize biofuel production commercially. By using biosensors, we hope to provide an accurate, cheaper, and less time-consuming method of assessing biofuel production.
About the image: Our current system uses a two plasmid system that requires both arabinose and the desired analyte to produce RFP. Using an arabinose inducible promoter ultimately allows better control of the transcription of RFP. |
