Resources

Advice and Best Practices for Future iGEM Teams

Original research rarely goes according to plan. That means many experiments will fail. Try to split your project into phases that can be completed in parallel.

Be very careful when ordering DNA synthesized by a third party. Especially be ware that by default you will be shipped single-stranded DNA, so you must specify "duplex" if you need double-stranded DNA.

External Links

Tools

Reverse and/or Complimentary Sequences - Free, browser-based applet for manipulating sequences according to IUPAC conventions

Serial Cloner 2.1 - Free, light molecular biology utility

Gene Designer 2.0 - Free, robust sequence design application

Papers

Refinement and standardization of synthetic biological parts and devices - Canton, Labno, and Endy (2008)

Can Patents Deter Innovation? The Anticommons in Biomedical Research - Heller and Eisenberg (1998)

Polymeric system for dual growth factor delivery - Richardson, Peters, Ennett, Mooney (2001)

Angiogenic effects of sequential release of VEGF-A165 and PDGF-BB with alginate hydrogels after myocardial infarction - Hao, Silva, Månsson-Broberg, Grinnemo, Siddiqui, Dellgren, Wärdell, Brodin, Mooney, and Sylvén (2007)

Spatiotemporal control of vascular endothelial growth factor delivery from injectable hydrogels enhances angiogenesis - Silva and Mooney (2007)

Mechanisms of angiogenesis and arteriogenesis - Carmeliet (2000)

Synthetic Extracellular Matrices for Tissue Engineering and Regeneration - Silva and Mooney (2004)