Results

BioBrick assembly results

In order to test the BrickMason assembly method, we decided to build the following construct:

Figure 1. Construct created by BrickMason assembly. It is composed of 6 parts, the parts will be referred to by the red numbers shown above them.

1. Part Size (bp)

1 Ade2 up 234 2 KanMX 1424 3 TDH3 725 4 Ura3 801 5 GFP 714 6 Ade2 Down 288

12 Ade2 up - KanMX 1658 23 KanMX - TDH3 2149 34 TDH3 - Ura3 1526 45 Ura3 - GFP 1515 56 GFP - Ade2 Down 1002 Table 1. The number, name, and size of each part used in the construction. The top half of the table lists information for monomers, and the bottom half lists information for heterodimers.