From 2011.igem.org
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TITLE=Building a Naphthenic Acid Biosensor: Summary|
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The Design
[[Image:UofC2011_DataPage1.png|thumb|600px|center|
Figure 1 In order to develop a naphthenic acid biosensor we were required to develop a sensory element which would respond specifically to this class of compounds. Additionally, we developed a novel electrochemical reporter system using the lacZ gene product Beta-galactosidase to detect and quantify the response of our promoter into a measurable system. Since
E. coli is not naturally found in tailings ponds, we designed a novel conjugation system to transfer our reporter system into
Pseudomonas as well as protocols for use of our system in microalgae.
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