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Chloramphenicol stock
From 2011.igem.org
Materials:
Chloramphenicol powder
ddH2O
sterile syringe
syringe filter
sterile 15ml centrifuge tube
Procedure:
1. Weigh the appropriate amount of antibiotic powder for the amount of ddH2O to be used. (we use 0.165g in 5 ml of H2O to make a 33mg/ml solution.)
2. Mix your antibiotic with your ddH2O
3. Absorb your solution with the sterile syringe.
4. Place the syringe filter onto the syringe.
5. Filter your solution into the sterile 15ml centrifuge tube.
