Team:WHU-China/Notebook

• Dealing with biobricks • Preparation of competent cells (Using Calcium Chloride) • Plasmid Extraction（Using Plasmid Mini Kit） • Transformation (heat-shock method) • Cleavage with two restriction enzymes • Get Extraction • Identification of DNA by Electrophoresis • Ligation • PCR of the colony • Identification of DNA by Sequencing • Others............

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