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Team:UNAM-Genomics Mexico/Notebook/pBBR1MCS-5
From 2011.igem.org
Contents |
Lab Logbook -pBBR1MCS.
Week: june 13 to 18
At beginning of the week was decided the best way to insert a prefix-RFP-suffix fragment to thepBBR1MCS-5 plasmid, which is a plasmid with broad host-range and is capable to conjugate to R. etli through E. coli S17, being the most efficient way to introduce genes into R. eltli. The pBBR1MCS-5 has a multiple cloning site (MCS) with the following restriction sites: KpnI ApaI XhoI Sal I Bsp106 I ClaI HindIII EcoR1 PstI SmaI Bam H1 SpeI XbaI BstXI SacI
