"

From 2011.igem.org

Contents 1 Sunday 1.1 Mutagenesis of Xyle, Take 3 Day 4 2 Monday 2.1 Insert tev Protease into K3 Vector, Day 4 2.2 Insert Imperial Linker into K3 Vector, Day 4 2.3 Mutagenesis of Xyle, Take 4 Day 1

Sunday

Mutagenesis of Xyle, Take 3 Day 4

     The PCR product of the mutated XylE gene from the 4°C fridge was purifed, transformed into Escherichia coli cells and plated onto ampicillin resistant plates.

Monday

Insert tev Protease into K3 Vector, Day 4

     Sequencing Results: The tev protease sequence came back 99% correct. The assembly features a single point mutation at base pair 515: TCT (serine) -> CCT (proline).

Insert Imperial Linker into K3 Vector, Day 4

Since we failed to make freezer stock of this correctly assembled part, we transformed the Imp linker + K3 miniprep made for sequencing on Friday, 6/10 into Escherichia coli cells and plated onto kanamycin resistant plates.

Mutagenesis of Xyle, Take 4 Day 1

Back to Notebook