Data Collection

To collect our data, we first cultured our samples in a 96-well plate for six hours at 37 C, or 12 hours at room temperature. This allowed us to ensure that all of our samples were in exponential phase at the start of the experiment, and that the growth curves would be synchronized across wells.

We then filled a second 96-well plate with media of various conditions, and inoculated with 15 uL of culture from the culture plate. We were sure to include both positive (Wildtype promoter) and negative (LB, DH5alpha) controls.