From 2011.igem.org
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Week 1
| 7/11/2011 |
Preparing sent parts
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7/12/2011
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Preparing sent parts
growing LB and appropriate antibiotic overnight
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7/13/2011
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all grew, negative controls didn't
Preparing sent parts, take 200µL from LB grown overnight and plated on appropriate antibiotic
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7/14/2011
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all grew a blob and a few colonies along the edge
Transformation at 37 degrees Celsius overnight- 50 µL DH5a, 2 µL DNA (all carb)
Plating sent parts
Take one colony from July 13 plate properly
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7/15/2011
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Transformation of 50µL of DH5a, 2 µL DNA (all carb) at 37 degrees overnight
Incubated in 3mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm. |
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