| 7/25/2011 |
Incubate LB chlora for 1 hour
Negative control continued overnight
Test Experiment #1- Took 10 µL from i2.9.7 and 10 µL from i2.9.8 and mixed them together.
Then plated all 20 µL onto a chlora plate.
Then we covered it and LED lights in the 37 degrees Celsius incubator and let them grow overnight.
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7/28/2011
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i2.10.1 - FOFR from commercial stock, i2.10.2- negative control, i2.10.3-agar stab being plated on carb plate
Incubation FOFR colonies (3 mL of LB and carb)
Plating mutated CheZ (inoculation)
grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB
grew FOFR (fibroblast growth factor receptor) which was on pCMV- SPORT6.1 in E.coli strain DH1OB
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7/29/2011
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Making glycerol stock
Miniprep
Observations:
i2.10.3 grew, i2.10.1 grew, i2.10.2 didn’t grow
Sore i2.10.5 until primers come in, then proceed to PCR, gel electrophoresis/purification, digest, gel electrophoresis/purity
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7/31/2011
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Incubation of CheZ (I2IT)- 3mL of LB and carb |
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