Team:Minnesota/leex4462-05242011000000-45

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Miniprep and PCR, Liquid Cultures

Project: Surface Display

Date: Tue, 24 May 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

Tue, 23 Aug 2011 12:02:18 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

-Miniprep and PCR liquid culture to test whether the ompA insert was successfully inserted into the pBAD plasmid

-The 2nd liquid culture was a backup in case the results show negative (no ompA insert).

Procedures:

-Miniprep as usual

-Used ompA primers to test miniprep DNA using PCR

-Started 2 liquid cultures from colonies of the 2nd transformation.

Results:

-Miniprep concentration=30.1 ng/ul

-The liquid cultures failed


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