Team:Minnesota/davis950-09092011121301-119

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== Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8 == Project: Regulatory Date: Fri, 09 Sep 2011 12:13:01 GMT Author: Nathan Davis (davis950) Access: Public Revision History:

Fri, 09 Sep 2011 12:19:11 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)
Fri, 09 Sep 2011 12:19:30 GMT (davis950): entry data updated by davis950 (id=2)

Purpose: to digest pUC-pcyA-ho1 and pUC-pTet-Cph8 with appropriate restriction enzymes to put pTet-Cph8 in pUC-pcyA-ho1. Procedure: The following digestion reactions were prepared:

16 ul ddH2O

1 ul SpeI

1 ul PstI

3 ul NE buffer 2

10 ul pUC-pcyA-ho1 miniprep product (139 ng/ul)

16 ul ddH2O

1 ul XbaI

1 ul PstI

3 ul NE buffer 2

10 ul pUC-pTet-Cph8 (105 ng/ul)

Digest reactions placed in 37 degree C incubator overnight. Results: None


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