Team:UT Dallas/week7
From 2011.igem.org
Week 7
8/21/11 | Incubation: GFP |
8/22/11 | Glycerol Stock/Miniprep GFP Test Digestion + Gel Electrophoresis: GFP |
8/23/11 | Full Digestion: CheZ, CheZ*, ctx promoter Dissolving/Diluting Primers: ----------------------------- |
8/24/11 | PCR Point Mutation: FGFR (get rid of EcoRI restriction site) Transformation (XL10 ultra competent): FGFR (point mutated) |
8/25/11 | Incubation: FGFR
Digestion, Gel Electrophoresis/Purification: CheZ, CheZ* |
8/26/11 | Glycerol Stock/Miniprep: FGFR Transformation CheZ, CheZ* |
8/27/11 | Digestion, Gel Electrophoresis/Purification: RBS-TetR, GFP Ligation (10 min): GFP into RBS-TetR-double term |