Team:Calgary/Notebook/Protocols/Process14

Biobrick parts are shipped from the registry in a dehydrated from. As such they must be rehydrated before they can be used.

1. Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
2. Add 15 μL of diH20 (deionized water)
3. Let the water sit for 5 minutes
4. Take 2 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies