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From 2011.igem.org

{{Team:Calgary/Notebookbar|

TITLE=RNA Extraction from Pseudomonas spp.| BODY=

Reagents

1. TE buffer (10 mMTris•Cl, 1 mM EDTA, pH 8.0)containing 1 mg/ml lysozyme
2. Buffer RLT with beta-mercaptoethanol (10μL/1mL of RLT)
3. Buffer RPE with 95% ethanol (4 volumes ethanol to 1 volume ethanol)
4. RNAprotect Bacterial Reagent
5. 95% ethanol
6. RNeasy Mini Columns
7. Genome Trap Columns
8. Buffer RW1
9. Buffer RDD
10. DNase I
11. RNAse Free Water

Procedure