"
Team:UEA-JIC Norwich/Notebook
From 2011.igem.org
Lab Journal
Monday 20th June - Attempted transformations of E.coli with biobricks for the first time. We used the Banana odour generator; red and green luciferase; and Epic biobricks. We followed the protocol offered by the New England Biolabs for this procedure (see methods). We find out tomorrow if it worked!
Tuesday 21st June- The control cells and 'Epic' Biobrick cells were successfully transformed. The cells transformed with the 'Banana odour' Biobrick mysteriously vanished from the incubator and were found a day later in the chill room. All other transformations failed. We repeated protocols of all unsuccessful transformations.
Wednesday 22nd June- All transformations successful. Much relief. Made cultures from colonies of all transformations with the aim of making glycerol stock solutions as soon as possible.
Standard Journal
Wednesday 22nd June - Told last night after going to see X-men that we need to have a poster completed and ready for use by friday. Spent the day writing poster drafts and designing the layout, as well as attempting to copy the logo we designed onto the computer.
