Team:MIT/Results/

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  • DNA Delivery
  • Parts Constructed
  • Parts Characterizations
  • Patterning Modeling Results
  • Patterning Experimental Results
  • Cell Adhesion Results
  • Attributions

DNA Delivery Systems

PUT STUFF HERE.

Parts Constructed

Name: Hef1a-LacOid MammoBlock ID: BBa_K511000
Type: Regulatory Length: 1275

This part encodes a promoter with low-level, constitutive activity that can be repressed by variants of the LacI transcriptional repressor. Repression by LacI-KRAB through chromatin packing is quite effective.

Description/Usage:
Characterization Link
ADD MORE PARTS WITH SAME FORMAT

Parts Characterizations

The characterization of newly constructed biological parts is ADD TO BLURB

List of characterizations

rtTA3/TRE promoter

Explanation:

EXPLAIN HERE

Gal4/UAS promoter

Explanation:

EXPLAIN HERE

LacI/Hef1a-LacO repressor

Explanation:

EXPLAIN HERE

Delta-Notch interaction

Explanation:

EXPLAIN HERE

CMV-TetO promoter

Explanation:

EXPLAIN HERE

Mnt-VP16/Mnt promoter

Explanation:

EXPLAIN HERE

CI434-VP16/CI434 promoter

Explanation:

EXPLAIN HERE

Gal4→LacI¬rtTA3→Reporter cascade

Explanation:

EXPLAIN HERE

Patterning Modeling Results

Semon's Modeling Results goes here

Patterning Experimental Results

PUT STUFF HERE for Notch-Delta Experimental Results

Cell Adhesion Experimental Results

PUT STUFF HERE for Cell Adhesion

Attributions

Our instructors were very helpful not only in giving feedback on our designs, cloning strategies, and data, but also in training us for lab work. The training for tissue culture work was conducted by Linda Stockdale in the Griffiths lab. Gibson assembly techniques and FACS training from Deepak Mishra, one of our instructors.

Other than the initial training, all work was done by our undergraduate team.

Special credit belongs to Semon Rezchikov and Jenny Cheng for simulations and modeling, and Tiffany Huang for wiki design.