Team:MIT/Results/
From 2011.igem.org
Parts Constructed
Name: Hef1a-LacOid MammoBlock
ID:
BBa_K511000
Type: Regulatory Length: 1275
Characterization
Type: Regulatory Length: 1275
Description/Usage:
This part encodes a promoter with low-level, constitutive activity that can be repressed by variants of the LacI transcriptional repressor. Repression by LacI-KRAB through chromatin packing is quite effective.
Characterization
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Parts Characterizations
The characterization of newly constructed biological parts is ADD TO BLURB
List of characterizations
- rtTA3/TRE promoter
- Gal4/UAS promoter
- LacI/Hef1a-LacO repressor
- Delta-Notch interaction
- CMV-TetO promoter
- Mnt-VP16/Mnt promoter
- CI434-VP16/CI434 promoter
- Gal4→LacI¬rtTA3→Reporter cascade (!)
rtTA3/TRE promoter
Explanation:
EXPLAIN HERE
Gal4/UAS promoter
Explanation:
EXPLAIN HERE
LacI/Hef1a-LacO repressor
Explanation:
EXPLAIN HERE
Delta-Notch interaction
Explanation:
EXPLAIN HERE
CMV-TetO promoter
Explanation:
EXPLAIN HERE
Mnt-VP16/Mnt promoter
Explanation:
EXPLAIN HERE
CI434-VP16/CI434 promoter
Explanation:
EXPLAIN HERE
Gal4→LacI¬rtTA3→Reporter cascade
Explanation:
EXPLAIN HERE