Team:Caltech/Week 2

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Caltech iGEM 2011

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June 19

Collection of soil and water samples from LA River

June 20

Lab walkthrough
Setting up lab, stock supplies, preparation of LB-amp plates
Test extraction of DNA from BioBrick wells into cloning plates
Enrichment cultures of collected samples on minimal media with BPA

June 21

MoBio kit extraction of DNA from collected samples
Enrichment cultures of samples in minimal media with 17α-estradiol, nonylphenol, and DDT
Geneious and primer design tutorial

June 22

Miniprep and sequencing of selected BioBricks (K123000, K123001, K123002, K123003)
Preparation of competent cells

June 23

PCR, digest, ligation and transformation of human estrogen receptor BioBrick to remove intron
Start cultures of RFP, transformed HER
Design and order primers for the test sequence