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Team:Northwestern/Notebook/Week11
From 2011.igem.org
PROJECT
RESULTS
CONSIDERATIONS
ABOUT US
NOTEBOOK
ATTRIBUTIONS
Notebook
Week 11
Day 49 - Monday, August 22nd 2011
- More extensive minipreps - this time of all the genomic promoter ligations.
- Plated our glycerol stocks of the successful gels from friday to make sure everything worked properly.
- Ran a plate of our controls at the high-throughput lab.
- Digested and ran the genomic promoter ligations on a gel
Day 50 - Tuesday, August 23nd 2011
- Analyzed the results from our control plate at the high throughput lab.
- We wanted to continue testing, but we were having trouble getting all of our samples to grow properly.
- Prepared 384 well plate layouts for future tests.
Day 51 - Wednesday, August 24nd 2011
- The bacteria continued to have very weird growth patterns. We tried two other spectrophotometers to try and judge if ours was malfunctioning.
- We eventually decided that our machine was okay, but that we had not been mixing the samples properly before reading them, which led to our weird results.
- We made new M9 media in case that was causing the growth problems.
Day 50 - Thursday, August 25nd 2011
- Ran a 96 well plate with our controls and GFP samples. We added the autoinducer to all of the samples at an OD of approximately 0.3
- Began setting up interviews and a script for our human practices video
Day 51 - Friday, August 26nd 2011
- Analyzed the results from our GFP test.
- Continued working on human practices
