Project Hunger

Production of needless enzymes is a heavy burden especially when the resource is scarce. This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC^† which activate σ⁵⁴promoter when the supply of nitrogen is not enough. NtrB and NtrC are coded in glnL and glnG, respectively. Here, we evaluated the relationships between expression under σ⁵⁴promoter and that of these genes by the aid of RPU(a relative promoter unit).

† NtrB and NtrC are otherwise called NR_II, NR_I

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Introduction

For every living thing, needless biological activity is not efficient. Cells must be controlled so that enzymes are produced only when they are necessary. Ammonia is an essential nitrogen source for the bactria. When enteric bacteria are deprived of ammonia, they express glnA to produce glutamine synthetase(GS). Nitrogen is used in the reaction of

Glutamate + NH₃ + ADP  →  Glutamine + ADP + phosphate
                       GS

The expression of glnA is regulated by several proteins including NtrB, NtrC, Pii.

Fig. NtrCがσ54,RNAポリメラーゼ,DNAの関係図(窒素源あり/なし)
Fig. NtrBCの関係図
Fig. Ntr, Pii, GSの関係図

Team:Kyoto/Hunger

From 2011.igem.org

Contents

Project Hunger

Introduction

Method

Result

Discussion

Reference