Week 13:
09/13/2011
1. Check quality of gDNA
2. Make HT115 + BL21 (tetO, CRISPR-GFP #7) competent cells, freeze stock
3. Miniprep PCDF+casO
C. Plate 4 Well 16O (LovTAP Composite)
4. Digest tetO/tetR
09/15/2011
1. Run a gel of all minipreped PCDF+casO
2. PCR HT115, DH5α2, DH5α3 with primers
09/18/2011
1. Inoculate the following:
BL21 tetO::GFP
2. BL21 tetO::GFP, CRISPR::GFP#7
3. BL21 tetO::GFP, CRISPR::GFP#7, cas3
4. HT115 tetO::GFP
5. HT115 tetO::GFP, CRISPR::GFP#7
6. HT115 tetO::GFP, CRISPR::GFP#7, cas3
7. HT115 CRISPR::GFP #7
C. Plate 4 Well 16O (LovTAP Composite)
D. Plate 1 Well 2B (GFP+PEST191)
E. Plate 2 Well 4A (Yeast ADH1 promoter)
06/10/2011
1. Freeze the inoculated culture
2. Observation: BBa-I15008 and BBa-I63009don’t have as much growth as others
06/11/2011
1. Purify plasmid DNA from the culture
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