Team:UC Davis/Data LambdacI

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LacI Promoter Characterization Data

Wildtype

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.99315, 0.78019, 0.58405, 0.45400, 0.35401, 0.29099, 0.13305, 4 0, 1, 2, 5 0.00000, 0.20000, 0.40000, 0.60000, 0.80000, 1.00000, 2.00000 0.99315, 1.02097, 0.99023, 0.99354, 0.78019, 0.97021, 0.96725, 0.96226, 0.58405, 0.87100, 0.89795, 0.94529, 0.45400, 0.76333, 0.85071, 0.93618, 0.35401, 0.65243, 0.75444, 0.88056, 0.29099, 0.55454, 0.68266, 0.84441, 0.13305, 0.26829, 0.37015, 0.55294

Mutant 1

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.68829, 0.48655, 0.27198, 0.15260, 0.08426, 0.05322, 0.01741,

Mutant 2

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.68514, 0.43727, 0.27724, 0.17817, 0.11684, 0.08021, 0.03415,

Mutant 3

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.67026, 0.44322, 0.28504, 0.17543, 0.10938, 0.07325, 0.02937,

Mutant 4

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.31888, 0.16625, 0.10228, 0.05821, 0.03985, 0.02549, 0.01329,

Mutant 5

caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.63174, 0.33524, 0.17440, 0.09337, 0.03043, 0.03337, 0.01686,

Mutant 6

caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.42290, 0.29347, 0.15733, 0.09471, 0.05778, 0.03850, 0.01475,

Mutant 7

caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca 0, .0014, .0028, .0042, .0056, .0070, .0140 0.58989, 0.32402, 0.20047, 0.11183, 0.06365, 0.03915, 0.01712,
Some preliminary data. These are LacI promoter mutants. The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter. There's still more to come!