Team:WashU/Notebook/Transformation
From 2011.igem.org
Transformation Group
The transformation team worked on the bacterial transformation. The team took any DNA plasmid and transforms the plasmid into the bacterial genome. We then cultured the transformed bacteria and isolate the DNA plasmid. This procedure amplifies the amount of DNA plasmid.
The team also transformed DNA plasmid into yeast genome. After the microbiology group ligate the genes in the plasmid, we were responsible for inserting the genes into yeast. We were responsible for sporulation of the yeast with different gene insertions to produce daughter cells with both genes of interest.