• 
• Home
• Project
  • Hunger
  • Hunger Modeling
  • Capture
  • Digestion
  • Digestion Modeling
• Consideration
  • Safety
  • Human Practice
• Method
  • Parts
  • Protocol
  • Material
• Team
• Notebook
  • Lab Work
  • Diary
• Acknowledgement
• Criteria

Project Luminescence

The Phototaxis of Drosophila melanogaster against lights of different wavelengths from --nm to --nm and weak luminescence emitted by Luxbrick(BBa_K325909, iGEM Cambridge2010) were evaluated. Drosophila melanogaster was chosen as the object for our research because of its popularity among Japanese houses, facility or easiness to breed and genetical identity which results in the similar behavior. From the result of the research, Drosophila melanogaster was demonstrated to be a good ammoniacal source for genetically modified bacteria.

Introduction

In the nature, there are some organisms which lure insects. Rafflesia[http://en.wikipedia.org/wiki/Rafflesia] attracts flies by distinctive corrupt smell like rotting flesh. Pyrearinus termitilluminans, which makes and lives in a tunnel around the eternal surface of the anthill of termite Cornitermes cumulansand, emits light in the first week of the rainy season to lure and hunt the termites. Arachnocampa also uses light to hunt flies. For Carnivorous E.coli, we chose light, like Pyrearinus termitilluminans, as the method to lure insects. The Luxbrick created by the iGEM 2010 Cambridge team (BBa_K3225909) emits fluorescense.

===Our works=== To probe that Carnivorous E.coli can lure insects. we, group luminescence, intend to do the following things. *Confirmation that drosophila, our model organism, have positive phototaxis in blue light (E.glowli created by 2010 Cambridge Team emits blue light) *Confirmation that drosophila moves to light emitted by E.coli 

The flowers look and smell like rotting flesh

Materials and Methods

1. Apparatus

Y maze

Y maze was constructed by the commonly available straws,__ , LEDs and __ to clamp LEDs. Straws were _色_ striped, in diameter and made of _. _ was made of metal, the angles of Y-form was _ in its top and _ in its side. LEDs were ,, and. __s were cut out of _ into re-entrant shape whose detailed dimension is described below in the figure. After preparing all in above they are connected together with tape and put into the dark room.

ここに装置全体の写真とイラストを載せる。

↑なし/ Diagrammatic representation of the Y maze equipment constructed from straws and __.

ここにLED固定用の装置の寸法を記したイラスト/写真を載せる

Pooter(吸虫管)

Straw was constructed by the commonly available straw, plastic tip and tissue. The tips were used as mouthpiece and objective end.

ここに装置全体の写真とイラスト(完成図と制作方法)を載せる。

↑なし/ Diagrammatic representation of the pooter constructed from a straw, pipets and tissue.

2. Object

The flies were derived on _日付_ August 2011 from the _strain_ of Drosophila melanogaster kept in _場所_. They were maintained in the temperature controlled incubator at _℃ with a _h light phase(LD 時間:時間) and cultured in the ordinary glass bottle (cm high, cm in diameter)with _培地の名前_medium. The medium was produced according to the method in http://. Every day, the newly born flies were transferred to another bottle to control the postnatal day. The population size of the flies were between _to_.

3. Method

ハエオス/メス○匹ずつを吸虫管

Result

Discussion

Reference

1. Rafflesia. In Wikipedia Retrieved 6 September 2011 at 16:02., from http://en.wikipedia.org/wiki/Rafflesia[1](※引用の方法あってるのか自信がない)