Team:Kyoto/Protocol

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Protocol

Medium for drosophila

[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Medium for drosophila]
Materials Methods
  • water : 500mL
  • dry yeast : 20g
  • corn flour : 45g
  • glucose : 50g
  • agarose : 3.5~5g
  • propionic acid : 1.5mL
  • 10% p-hydroxybenzoate in 70% Eternol : 5g
  1. Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
  2. Stir corn flour and glucose with the remaining water.
  3. Stir 1 and 2, then autoclave it again.
  4. after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■




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