Team:Paris Liliane Bettencourt/Notebook/2011/08/20/

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Babak

Antibiotics experiment

All mixtures were spotted on LB+IPTG plated at 37 degrees and then replicated on plate containing both antibiotics, chloramphenicol and Kanamycin.

• M1 is a mixture of SB463 bacillus strain, harboring chloramphenicol resistance, and SB513 B.subtilis strain, which is resistant to Kanamycin.

• M2 is a mixture of SB463 bacillus strain, which is resistant to chloramphenicol, and K12 E.coli strain (S33), which is resistant to Kanamycin.

• M3 is a mixture of K12 E.coli strain (S34), harboring chloramphenicol resistance, and SB513 B.subtilis strain, which is resistant to Kanamycin.

• M4 is a mixture of K12 E.coli strain(S34), harboring chloramphenicol resistance, and K12 E.coli strain (S33), which is resistant to Kanamycin.

• SB 463, SB 513, S34 and S33 are negative controls

Antibiotic Experiments all mixtures

We use 6µg/mL concentration of both Antibiotics and 100µg/mL of IPTG on LB Agar plate.