green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Miniprep
Investigators: Sophie
continue from: Transformation, 19.08.11
Testdigest showed, that cloning was not succesful. Seems that the vector was only partly digested, because only vector-bands were visible on gel. I will try it again with longer digestion times.
Miniprep
Investigators: Rüdiger
Miniprep of PET.DUET_1 vector.
Trafo
| Name:Rüdiger
| Date:20.08
|
| Continue from Date 19.08. Name Rüdiger
Experiment Ligation
|
| Project Name: Precipitator
|
Procedure
- take cells from -80°C freezer and put them on ice! (every eppi contains about 400 μl cells)
- thaw cells on ice 20 minutes
- pipette 50 μl cells and 2 μl DNA into eppi still on ice!
- Incubate for 30 minutes on ice
- Heat at 42°C for 60 sec
- Incubate on ice for 5 minutes
- Add 200 μl LB Broth
- Incubate for 2 hours at 37°C (cells with lysis cassette at 30°C!!)
- Plate 50 μl and 200μl on two different LB/Agar plates with appropriate antibiotic resistance
Documentation:
Why are you doing this experiment? Name of the sample? Where are they stored? Name the vector with inserts, antibiotika resistance etc.
| Trying to get Precipitator into iGEM vector.
Did not work.
|
Investigators: Rüdiger
Transformation of precipitator(pSB1C3).
Trafo did not work.
10.09.
Rüdiger
Picked cells from yesterday's transformation
1 a/b A-C
2 a/b A-C
3 a/b A-C
5 a/b A-C
6 a/b A-C
7 a/b A-C
-miniprep of yesterday's inoculation.
11.09.
-miniprep of yesterday's inoculation.
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