green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
Ligation
Investigators: Sandra
Ligation of LovTAP, Not-Gate in pSB1T3.
Stored in the freezer in Gibson stuff.
Next step: Transformation
red light receptor
MIniprep
Investigators: Sandra
Miniprep of:
- 1pTd 1/2 (PR1+pcyA+terminator)
- 2pTd 1/2(PR2+pcyA+terminator)
- 1pTb 1/2(PR1+pcyA+terminator)
- 2pTb 1/2(PR2+pcyA+terminator)
- 1L23 1/2(PR1+ho1+terminator)
- 2L23 1/2(PR2+ho1+terminator)
and S54a,b,c,d.
| Sample
| DNA concentration
|
| M54a
| 181.0
|
| M54b
| 224.9
|
| M54c
| 222.0
|
| M54d
| 205.9
|
| 1pTb1
| 27.2
|
| 1pTb2
| 17.9
|
| 2pTb1
| 33.2
|
| 2pTb2
| 27.6
|
| 1pTd1
| 40.9
|
| 1pTd2
| 33.6
|
| 2pTd1
| 37.9
|
| 2pTd2
| 37.7
|
| 1L231
| 15.3
|
| 1L232
| 37.3
|
| 2L231
| 25.2
|
| 2L232
| 24.1
|
stored in the undigested miniprep box III.
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Miniprep
Investigators: Rüdiger
Miniprep of pGex-GP-1.
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