green light receptor
Results Transformation
We dont see any colonies
- To-do: do quickchange again.
Quickchange
Investigator:Jakob
PCR
| Name:
Jakob
| Date:
09.08.2011
|
| Continue from Experiment:
|
| Project Name:
Quickchange CcaR and CcaS
|
PCR-Mixture for one Reaction:
For a 50 µl reaction use
| 32,5µl
| H20
|
|
| 10µl
| 5x Phusion Buffer
|
|
| 2.5µl
| Primer fw
| Primer: P12(CcaR) and P14(CcaS
|
| 2.5µl
| Primer dw
| Primer: P13(CcaR) and P15(CcaS)
|
| 1µl
| dNTPs
|
|
| 1µl
| DNA-Template
| DNA: CcaR and CcaS
|
| 0.5 µl
| Phusion (add in the end)
|
|
- And again, I'm such a fool...
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
Lysis cassette sequencing
Investigators:Theo
Sequencing showed an IE (insertion element) or part of the E.coli genome inside the lysis cassette (aaargh)
File:Lys1-P8.gb
Precipitator
new 3A assenbly with Amp Vector
Investigators: Sophie
3A assembly
The last cloning of Theo and Jakob produced one clone with GFP-plastic-binding-domain (GFP-pbd) inside but without Promoter-Ribosome-binding site (PR). So I tried again to combine the GFP-pbd with PRs via 3a assenbly. I used two different PRs (PR4 and PR6) with different RBS binding strenght.
Name of the samples:
ε5 (GFP-pbd),
pSB1A3,
PR4,
PR6.
stored in "Minipreps, verdaut"-box
Ligation
I ligated ε5 (GFP-pbd) to PR4 or PR6 and to the Amp-Vector. Therefore I once took the ratio 3:1 insert to vector and once the ration 1:1 insert to vector.
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