Team:Glasgow/Results/PromoterLibrary/Results

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Results:

Stock of Promoters and Ribosome Binding Sites
We have successfully created a stock of all the promoters and ribosome binding sites which we aimed to make.

Stocks of Ligated Constructs

We have successfully obtained colonies from the following ligations:

Strong Promoter + Strong RBS
Strong Promoter + Weak RBS
Weak Promoter + Strong RBS
Weak Promoter + Weak RBS
pBAD Promoter + Strong RBS
pBAD promoter + Weak RBS
Bluf Promoter + Strong RBS
Bluf Promoter + Weak RBS
OmpF Promoter + Strong RBS
OmpF Promoter + Weak RBS
OmpC Promoter + Strong RBS
OmpC Promoter + Weak RBS

These parts are all present in the plasmid which the promoter part was present in on the Registry.
Upon ligating the parts into the submission vector (pSB1C3), only the following constructs successfully grew and were confirmed to be the right size on a gel:
Bluf Promoter + Strong RBS
Bluf Promoter + Weak RBS
OmpF + Weak RBS
pBAD + Strong RBS
Weak Promoter + Strong RBS
Strong Promoter + Strong RBS
Weak Promoter + Weak RBS