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Team:Bilkent UNAM Turkey/Experiment
From 2011.igem.org
We ordered our genes. We optimized codon usage because of gene taken from E. cloacae.
We repeat this step a lot because of failuire.I put right result
We used standard gel purification kit Our ligation style is really similar to iGEM's one.
Our one;insert volume(µl):X
Cut Vector Volume(µl):X
DEPC-water(µl):8,5-2X
T4 DNA ligase(µl):0,5
Ligation Buffer(µl):1
Total Volume(µl):10
We transformed our plasmid into E.coli with a protocol.
We used invitrogen purification kit to get our plasmid. We sent genes to sequencing and sequencing data should be available on their link. PCR procedure for GFP amplification from pKScGFP For 1µl of DNA, we added, 1µl of forward primer 1µl of reverse primer
Codon Usage
Then we did restriction digestion and gel electrophoresis.We repeat this step a lot because of failuire.I put right result
We used standard gel purification kit Our ligation style is really similar to iGEM's one.
Our one;
We transformed our plasmid into E.coli with a protocol.
We used invitrogen purification kit to get our plasmid. We sent genes to sequencing and sequencing data should be available on their link. PCR procedure for GFP amplification from pKScGFP For 1µl of DNA, we added, 1µl of forward primer 1µl of reverse primer
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