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From 2011.igem.org

Tools for Pathway Engineering

It is easy to engineer a pathway. However, it is very difficult is tune protein expression levels within the pathway.

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There are many possible reasons why protein expression level can vary along a pathway. For example, if enzyme 2 were rate limiting, then the intermediate product B would build up within the cell. This is undesirable and a waste of resources.
In order to optimise this, the carbon flux needs to be rapid. To do this, you would need to be able to control the levels of each enzyme in the system.

This is why we have created a library of promoters with an RBS. This allows expression levels of enzyme to be readily controlled at each step in the pathway.
We have also created a Multiple Cloning site biobrick, which is ideal for use with our library of promoters.

These biobricks represent an invaluable set of tools for anyone working on expression levels in pathway engineering.

To read more about the creation of the Promoter + RBS library, click <a href=>here.</a> To read more about the creation of the Multiple Cloning Site, click <a href=>here.</a>