Team:Bilkent UNAM Turkey/Experiment

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Revision as of 21:10, 21 September 2011 by Ufuk (Talk | contribs)

We ordered our genes. We optimized codon usage because of gene taken from E. cloacae.

Codon Usage Then we did restriction digestion and gel electrophoresis.
We repeat this step a lot because of failuire.I put right result

We used standard gel purification kit Our ligation style is really similar to iGEM's one.
Our one;

  • insert volume(µl):X
  • Cut Vector Volume(µl):X
  • DEPC-water(µl):8,5-2X
  • T4 DNA ligase(µl):0,5
  • Ligation Buffer(µl):1
  • Total Volume(µl):10

  • We transformed our plasmid into E.coli with a protocol.
    We used invitrogen purification kit to get our plasmid. We sent genes to sequencing and sequencing data should be available on their link.