Team:Lyon-INSA-ENS/Realisation/Week7Fr

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Semaine 7


Du Lundi 25 Juillet au Vendredi 29 Juillet 2011







Lundi

Miniprep of some other parts from the transformed bacteria. Digestion and electrophoresis to control the presence of the right insert.

Miniprep of bacteria with mutated part CsgAB. Digestion to check restriction profile (removal of intern PstI site).





Mardi

Send of plasmid with the right profile for part CsgAB to sequencing. Extraction of new MC4100 strain's DNA. PCR with fresh DNA and Taq Phusion.





Mercredi

Les PCR n'ont pas marché.





Jeudi



Vendredi

La PCR a finalement marché avec la Phusion.








ENS assystem Biomérieux INSA INSA
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