Team:ITESM Mexico/Parts submitted
From 2011.igem.org
Part Registry
Name | Description | Type | Designer | Length | Lenght (prefix;suffix) |
BBa_K587000 | RecA | Protein coding | David Maycotte/Patricia Campos | 1062 | 1103 |
BBa_K587001 | Crxst | Regulatory | David Maycotte | 35 | 78 |
BBa_K587002 | Tast | Regulatory | David Maycotte | 80 | 123 |
BBa_K587003 | Tawk | Regulatory | David Maycotte | 79 | 122 |
BBa_K587004 | Crxwk | Regulatory | David Maycotte | 35 | 78 |
BBa_K587005 | Itast | Regulatory | David Maycotte | 81 | 124 |
== Sequences and description =='
*RecA
Sequence
GAATTCGCGGCCGCTTCTAGATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATT GAGAAACAATTTGGTAAAGGCTCCATCATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATAT CGCGCTTGGGGCAGGTGGTCTGCCGATGGGCCGTATCGTCGAAATCTACGGACCAGAATCTTCCGGTAAAACCACGCTGACGTTGCAGGTGATCGCCGC AGCGCAGCGCGAAGGTAAAACCTGTGCGTTTATCGATGCTGAACACGCGCTGGACCCAATCTACGCACGTAAACTGGGCGTCGATATTGACAACCTGCT GTGCTCCCAGCCGGATACCGGCGAGCAGGCACTGGAAATCTGTGATGCCCTGGCACGTTCTGGCGCAGTAGACGTTATCGTCGTTGACTCCGTGGCGGCAC TGACGCCGAAAGCGGAAATCGAAGGCGAAATCGGCGACTCTCACATGGGCCTTGCGGCACGTATGATGAGCCAGGCGATGCGTAAGCTGGCGGGTAACCTG AAGCAGTCCAACACGCTGCTGATCTTCATCAACCAGATCCGTATGAAAATTGGTGTGATGTTCGGTAACCCGGAAACCACTACCGGTGGTAACGCGCTGAAA TTCTACGCCTCTGTTCGTCTCGACATCCGTCGTATCGGCGCGGTGAAAGAGGGCGAAAACGTGGTGGGTAGCGAAACCCGCGTGAAAGTGGTGAAGAACAAA ATCGCTGCGCCGTTTAAACAGGCTGAGTTCCAGATCCTCTACGGCGAAGGTATCAACTTCTACGGCGAGCTGGTTGACCTGGGCGTGAAAGAGAAGCTTATC GAGAAAGCAGGCGCGTGGTACAGCTACAAAGGTGAGAAGATCGGTCAGGGTAAAGCGAATGCGACTGCCTGGCTGAAAGATAACCCGGAAACCGCGAAAGAG ATCGAGAAGAAAGTACGTGAGTTGCTGCTGAGCAACCCGAACTCAACGCCGGATTTCTCTGTAGATGACAGCGAAGGCGTAGCAGAAACTAACGAAGATTT TTAATACTAGTAGCGGCCGCTGCAG
*Crxst
This DNA sequence acts as a lock for the low arabinose concentration regulation path.
Sequence
GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTTGGATTTGGGTATCATACTAGTAGCGGCCGCTGCAG
*Tast
This sequence acts as the key for the Crxst lock and it unlocks it, in order to activate following mechanism.
Sequence
GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGAACCTAAAGGGATAGTT TACTAGTAGCGGCCGCTGCAG
*Tawk
This sequence acts as the key fot Crxwk lock and it unlocks it, in order to activate the following mechanism.
Sequence
GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGATAAACCGTAAGGGTT TACTAGTAGCGGCCGCTGCAG
*Crxwk
This DNA sequence acts as a lock for the high arabinose concentration regulation path.
Sequence
GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTATTTGGCATTCCCAATACTAGTAGCGGCCGCTGCAG
*Itast
This is a complementary sequence for the Tast key, so we call it the antikey since it will inactivate the Tast key and preventing it to interact with the Crxst lock.
Sequence
GAATTCGCGGCCGCTTCTAGAGTCTAGGAGAGATATATGGTATGGTGGTTAATTTTCATTAACCACCAAAGCGCAGTAATTCACCTCTTGGTTTCCCTATCAA TACTAGTAGCGGCCGCTGCAG