Part:BBa_K551001: pINDEL

The pINDEL plasmid is composed of 2 functional units:

1. the IN function which is composed of the gam, exo and bet genes coding for the lambda Red recombinase system
2. the DEL function which is based on the flp gene encoding the FLP site-specific recombinase and expressed at 42°C

Another feature of pINDEL is that its replication origin is not functional at 42°C due to the thermosensitivity of the RepA101Ts protein. This protein initiates replication at the ori site in permissive conditions (30°C) and is inactive at 42°C.
The deletion function and the thermosensitivity of the plasmid both could be shown to be working as intended (see functional parameters). The insertion function couldn't be properly tested