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Team:Groningen/project characterisation mu lasr
From 2011.igem.org
LasR Autoinducing Loops
Our second memory unit consists of a LasR gene(BBa_) driven the LasB promoter(BBa_). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.
| cI autoinducing loop 1 (BBa_K00) after 4 hours of incubation | |||||||||||||||||
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Figure 1. A dotplot of uninduced cells carrying the pSB1K3 plasmid with the biobrick BBa_ and the plasmid pSB1A3 with as well an input(PBAD controlling cI) as well as the output (PRM controlling GFP-LVA). |
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| Two populations are visible on the dotplot. We seperated both populations on the histogram to show that the major peak of nonfluorescent cells overlaps the minor peak of fluorescenting cells. | |||||||||||||||||
| cI autoinducing loop 6 (BBa_K00) after 4 hours of incubation | |||||||||||||||||
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Figure 2. A dotplot of uninduced cells carrying the pSB1K3 plasmid with the biobrick BBa_ and the plasmid pSB1A3 with as well an input(PBAD controlling cI) as well as the output (PRM controlling GFP-LVA). |
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| Only one population is visible on the dotplot. The histogram was obtained in the same fashion as in figure 1 to show that there are cultures with activated cI loops and cultures without. | |||||||||||||||||
