Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs

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Introduction
Our Project
Tools
- Gibson Assembly
- MITOMI
Notebook
- Protocols
- May
- June
- July
- August
- September
- October
Considerations
- Human Practices
- Safety
Acknowledgements
- Sponsors
- Partners

muTetRs

In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outline

Why

How

What

TetR variants

Mutagenesis

Promoter

Cterminal

DNA form

Expression

MITOMI vs 1-off

Biobrick

Sequenced

Sent to registry

V36F

site directed

SP6

eGFP

plasmid

worked well

v

BBa_K613013

PCR-induced

T7

His-tag

linear

(ins C-term), ITT tested

BBa_K613014

PCR-induced

T7

His-tag

linear

ITT tested

v

BBa_K613015

v

v

PCR-induced

T7

His-tag

template

ITT tested, worked

v

BBa_K613016

v

v

PCR-induced

T7

His-tag

linear

ITT tested

v

BBa_K613017

v

v

PCR-induced

T7

His-tag

linear

ITT tested

BBa_K613018

v

v

site directed

SP6

eGFP

plasmid

worked well

v

BBa_K613019

PCR-induced

T7

His-tag

linear

ITT tested

BBa_K613020

PCR-induced

T7

His-tag

linear

ITT tested

BBa_K613021

site directed

T7

His-tag

linear

BBa_K613022

v

site directed

T7

His-tag

linear

BBa_K613023

v

WT

none

T7

His&GFP

both

BBa_K6130ii

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